The first book of a two volume treatise on protein purification methods and applications, this title covers both traditional and recently developed techniques for initial purification, which is fundamental to any investigation of protein chemistry. The theory and basic principles of each technique are discussed, including initial planning, clarification and extraction, concentration of the extract, structure-based separation, activity-based purification, and gel permeation chromatography. Problem areas and solutions are highlighted at each stage, making this book equally valuable to novice researchers and more experienced protein biochemists who will find it a reliable and readily accessible bench manual.

The prediction of the three-dimensional structure of a protein from its amino acid sequence is a problem faced by an increasing number of biological scientists. This book provides a practical guide to making these predictions by reviewing strategies of different computer modeling algorithms and highlighting the degree of confidence attributed to them. Also in the volume are descriptions of the following: the principles of protein folding; sequence homology and motif searches; prediction of secondary structure; homology modeling; modeling of antibody combining sites; tertiary fold recognition; modeling of transmembrane proteins;聽ab initio聽prediction; protein-ligand docking simulations; and the use of molecular mechanics and dynamics. both non-specialists who require guidance to identify and evaluate appropriate strategies and experts who require a contemporary view of the field, will find this volume a worthy addition to their research libraries.